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projektai-lsd Lithuanian Standards Board
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prEN ISO 17601

Soil quality - Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soil (ISO/DIS 17601:2024)

Draft Public enquiry
Comment end date 2024-11-02
Export comments Read draft and comment

Project stage codes

1. Decision on WI Proposal
2. Projektas
3. Viešoji apklausa
From 2024-08-27 till 2024-11-02
4. Closure of enquiry
5. Submission to Formal Vote

Organisation

CEN Europos standartizacijos komitetas

ICS

13.080.30 - Biologinės dirvožemio savybės

Technical Committee

TK 57 Fertilizers

ForeignTC'S

CEN/TC 444

Number of comments

0

Comment start date

2024-08-27

Scope

ISO 17601:2016 specifies the crucial steps of a quantitative real-time polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract which provides an estimation of selected microbial groups. It is noteworthy that the number of genes is not necessarily directly linked to the number of organisms that are measured. For example, the number of ribosomal operon is ranging from one copy to 20 copies in different bacterial phyla. Therefore, the number of 16S rRNA sequences quantified from soil DNA extracts does not give an exact estimate of the number of soil bacteria. Furthermore, the number of sequences is not necessarily linked to living microorganisms and can comprise sequences amplified from dead microorganisms.