Microbiology of the food chain - Detection, isolation and characterization of Shiga toxin-producing Escherichia coli (STEC) - Part 1: Horizontal method for the detection and isolation of Shiga toxin-producing Escherichia coli (STEC) (ISO/DIS 13136-1:2024)

Comment end date 2024-05-03

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Project stage codes

1. Nauja darbo tema

2. Projektas

3. Viešoji apklausa

From 2024-02-27 till 2024-05-03

4. Closure of enquiry

5. Projekto priėmimas

Organisation

CEN Europos standartizacijos komitetas

ICS

07.100.30 - Food microbiology

Technical Committee

TK 51 Food analysis- Horizontal methods

ForeignTC'S

CEN/TC 463

Number of comments

Comment start date

2024-02-27

Scope

This standard describes the detection and isolation of Shiga toxin-producing Escherichia coli (STEC). The procedure includes the detection by Real Time PCR of the major virulence genes of STEC, stx1 and stx2 (Reference [2]) in enrichment cultures. Isolation of STEC from the enrichment culture is attempted if one or both stx genes are detected in the screening. This document is applicable to — products intended for human consumption, — products intended for animal feeding, — environmental samples in the area of food and feed production, handling, and — samples from the primary production stage.

Relations

Pakeis

prEN ISO 13136-1