prEN 15634-8
Foodstuffs - Detection of food allergens by molecular biological methods - Part 8: Peanut (Arachis hypogaea), hazelnut (Corylus spp.), walnut (Juglans regia) and cashew (Anacardium occidentale) - Qualitative detection of specific DNA sequences in food by real-time PCR
Draft Public enquiryProject stage codes
1. Decision on WI Proposal
2. Projektas
3. Viešoji apklausa
From 2026-01-30
till 2026-03-31
4. Closure of enquiry
5. Submission to Formal Vote
Organisation
CEN Europos standartizacijos komitetasICS
67.080.10 - Fruits and derived productsTechnical Committee
TK 51 Food analysis- Horizontal methodsForeignTC'S
CEN/TC 275Number of comments
0Comment start date
2026-01-30Scope
This document specifies a method for the qualitative detection of the species-specific DNA of peanut (Arachis hypogaea), hazelnut (Corylus spp.), walnut (Juglans regia) and cashew (Anacardium occidentale) in food of animal and plant origin, using real-time PCR, in the context of allergen analyses. The method was previously validated in an interlaboratory study (ring trial) and applied to DNA extracted from samples that consist of defined proportions of peanut, hazelnut, walnut and cashew in rice biscuits, cooked sausage, sauce powder, vegan cookie and veggie burger (powder). The limit of detection of each real-time PCR has been determined experimentally to be about 5 mg/kg (10 mg/kg for roasted peanuts).