prEN ISO 13136-2
Maisto grandinės mikrobiologija. Šigos toksiną gaminančių Escherichia coli (STEC) aptikimas, išskyrimas ir apibūdinimas. 2 dalis. Bendrasis išskirtų Šigos toksiną gaminančių Escherichia coli (STEC) apibūdinimo metodas (ISO/DIS 13136‑2:2024)
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Nuo 2024-03-12
iki 2024-05-17
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Organizacija
CEN Europos standartizacijos komitetasICS
07.100.30 - Maisto mikrobiologijaTechnikos komitetas
TK 51 Maisto analizėUžsienio technikos komitetas
CEN/TC 463Komentarų skaičius
0Komentavimo pradžia
2024-03-12Taikymo sritis
This document is applicable to pure cultures of STEC. The present standard describes the characterization of STEC strains, isolated from any source. In particular, the characterization of STEC strains described here regard: - subtyping of the Stx-coding genes; - determination of the presence of eae gene; - determination of the presence of aggR gene; - identification of the presence of genes associated to the following serogroups: O157, O26, O111, O103, O145, O121 and O45. The full characterization of the isolated STEC strains is achieved by performing all the modules described here. The characterization scheme is not sequential, and the different modules can be implemented separately based on specific needs (e.g. Regulatory needs, Competent Authority’s requests, clients’ request). Alternative methods may be used, including Whole Genome Sequencing (WGS), provided these are verified according to the reference standard ISO 16140-2.Ryšiai
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