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prEN 18338 Public enquiry
Comment end date 2026-06-09
Food authenticity - Sample preparation for isotope ratio analysis of fruit and vegetable juices and related products

This document describes a method for sample preparation for the determination of C, N and H isotope ratio values in different fractions (sugar and pulp) of fruit and vegetable juices and their derivatives (concentrates, nectars, beverages etc.) by Elemental Analyser-Isotope Ratio Mass Spectrometry (EA-IRMS) or Isotope Ratio Measurement-Deuterium Nuclear Magnetic Resonance Spectroscopy (irm-2H-NMR). It also covers ethanol produced by the fermentation of fruit and vegetable juices, and their derivatives. Sample measurement is not included within this document. This document does not concern the analytical methods after sample preparation, namely methods using IRMS (Isotope Ratio Mass Spectrometry) technique and isotope ratio measurement-deuterium nuclear magnetic resonance spectroscopy (irm-2H-NMR), also known as SNIF-NMR (Site-specific Natural Isotopic Fractionation by Nuclear Magnetic Resonance), used to quantify the isotopic ratios of the following nuclei: 13C/12C, 15N/14N and 2H/1H. The interpretation of the obtained isotope delta values is not covered by this document.

prEN 18337 Public enquiry
Comment end date 2026-06-09
Food authenticity - Determination of 18O/16O isotope ratios in liquid aqueous food matrices by Equilibration - Isotope Ratio Mass Spectrometry (Eq-IRMS)

This document specifies a method for instrumental analysis by equilibration-isotope ratio mass spectrometry (Eq-IRMS) of liquid, aqueous food matrices to determine ¹⁸O/16O isotope ratios of the water of the product. The ¹⁸O/¹⁶O isotope ratios obtained by following this document are expressed as δ¹⁸O values relative to internationally recognised reference materials. This document does not apply to sample preparation. It is assumed that the food sample has been pre-treated as necessary and homogenised. Similarly, the interpretation of the obtained δ¹⁸O values is not covered by this document. Following this protocol will result only in isotope delta values for the sample materials. Although other instrumental techniques can be applied to determine δ¹⁸O values in liquid, aqueous food materials, these other techniques are not covered by this document.

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