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prEN 18196 Viešoji apklausa

Komentavimo iki data 2025-08-12

Dumbliai ir jų produktai. Neorganinio arseno dumbliuose ir jų produktuose nustatymas anijonų mainų efektyviosios skysčių chromatografijos (HPLC) ir induktyviai susietos plazmos masių spektrometrijos (ICP-MS) metodu

This document describes a method for the determination of inorganic arsenic in algae by anion-exchange HPLC-ICP-MS following water bath extraction. Inorganic arsenic consists of arsenite, As(III) and arsenate, As(V). A representative test portion of the sample is treated with a diluted nitric acid and hydrogen peroxide solution in a heated water bath. Hereby the arsenic species are extracted into solution and As(III) is oxidised to As(V). The inorganic arsenic is selectively separated from other arsenic compounds using anion exchange HPLC (High Performance Liquid Chromatography) coupled on-line to the element-specific detector ICP-MS (Inductively Coupled Plasma Mass Spectrometry) for the determination of the mass fraction of inorganic arsenic. External calibration with solvent matrix-matched standards is used for quantification of the amount of inorganic arsenic. The method is based on method EN16802: Inorganic arsenic in food of plant and marine origin by HPLC-ICPMS, but covers more algae species. The present AsSugar species in certain algae can cause As peaks which might overlap with the As peaks related to the inorganic As. The current method includes a gradient elution method with quality criteria to ensure a correct identification of the inorganic arsenic.

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prEN 18197 Viešoji apklausa

Komentavimo iki data 2025-08-12

Dumbliai ir jų produktai. Mikrodumblių ir makrodumblių amino rūgščių profilio nustatymas

This document describes a method for determining the amino acid profile of algal biomass. It specifies a method for the determination, in one single analysis, of the following amino acids: alanine, arginine, aspartic acid (combined with asparagine), cystine (dimer of cysteine, combined with cysteine), glutamic acid (combined with glutamine), glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine and valine. This method does not apply to the determination of tryptophan. The existing draft standard ISO/DIS 4214 – Milk and milk products – Determination of amino acids in infant formula and other dairy products will be evaluated and adapted.

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